Antimicrobial evaluation, degree of solubility, and water sorption of universal dental adhesive incorporated with epigallocatechin-3-gallate: An In-vitro study

Abstract

Abstract Background: Several strategies have been developed to reduce collagen degradation in the adhesive interface. Epigallocatechin3gallate (EGCG) has the ability to stabilize collagen, and it is effective in microbial reduction. Aims and Objectives: The objective of the present study was to evaluate the antimicrobial potential, water sorption (WS), and solubility of a universal adhesive incorporated with EGCG at concentrations of 0.02%, 0.1%, and 0.5%, respectively. Materials and Methods: Atotal of 40 specimens of composite resin disks, to which the adhesives were applied, were divided into four groups: control (without EGCG), 0.02%, 0.1%, and 0.5%. The specimens were submitted to an in vitro cariogenic challenge, inoculated with Streptococcus mutans for 3 days for biofilm formation. The generated biofilm was collected, and the colonyforming units were established. For the solubility test, the specimens using the adhesive were divided into the same groups mentioned previously (n = 10). Adhesive models were made following an ISO standard for sorption and solubility tests. Microbiological data were submitted to the analysis of variance (ANOVA), followed by the Tukey test (P < 0.05). ANOVA was used to evaluate WS and solubility, and comparisons were made by post hoc analysis by the Student–Newman–Keuls method (P < 0.05). Results: A statistical difference was observed regarding the antimicrobial potential between the groups without and with EGCG at 0.5% (P = 0.03). EGCG 0.5% presented the highest values of solubility and WS (P < 0.01 and P = 0.009, respectively). Conclusion: The addition of 0.5% EGCG was capable of inhibiting biofilm formation; however, it caused significant alteration of the solubility and sorption of the adhesive.