Antimicrobial, antioxidant, anti-inflammatory activities and phytoconstituents of extracts from the roots of Dissotis thollonii Cogn. (Melastomataceae)

Abstract

BackgroundDissotis thollonii Cogn. belonging to the Malastomataceae family is used in the West Region of Cameroon for the treatment of inflammation, kidney diseases, pregnancy control and sinusitis. Despite the traditional use of this plant, no scientific report or information was found in the literature regarding neither its biological activity nor its chemical constituents. Aim of the studyThe present work was designed to determine the antimicrobial, antioxidant and anti-inflammatory activities of different extracts of the roots of D. thollonii Cogn. as well as the isolation and identification of the chemical constituents of this plant. Materials and methodsThe tests for antimicrobial, antioxidant and anti-inflammatory activities were performed over the MeOH, EtOAc, n-BuOH and aqueous extracts. Compounds were isolated from EtOAc and n-BuOH extracts of the roots of D. thollonii Cogn. through column chromatography and their structures were determined by means of NMR and MS analysis, and published data. ResultsAccording to the antimicrobial and antioxidant assays, the EtOAc and n-BuOH extracts were submitted to further separation and purification. This led to the isolation of twelve compounds identified as 3,3′-di-O-methylellagic acid 4′-O-β-D-xylopyranoside 1, 3-O-methylellagic acid 4′-O-β-D-arabinopyranoside 2, casuarinin 3, betulinic acid 4, β-sitosterol-3-O-D-glucopyranosyl-6′-mirystate 5, cellobiosylsterol 6, β-sitosterol 7, β-sitosterol-3-O-β-D-glucopyranoside 8, arjunolic acid 9, 3,3′-di-O-methylellagic acid 10, ellagic acid 11, and 3,3′-di-O-methylellagic acid 4′-O-β-D-glucopyranoside 12. The EtOAc extract was the only antimicrobial active sample [diameter of the zone of inhibition (DZI) of 10mm against Staphyloccocus aureus] among all the tested extracts. The analysis of fractions of this extract revealed the presence of bioactive compounds with a described antimicrobial activity such as β-sitosterol, β-sitosterol-3-O-β-D-glucopyranoside and arjunolic acid. By using Trolox as the standard drug, all extracts showed antioxidant activity against DPPH in the following order of scavenging ability: Trolox>nBuOH>EtOAc>MeOH>WE (water extract). The ABTS•+ scavenging ability was similar to that found for the DPPH assay, being Trolox>n-BuOH>MeOH>EtOAc>WE. Along with the DPPH and ABTS assays, the FRAP assay showed the scale n-BuOH>MeOH>WE>EtOAc. The phytochemical study of the EtOAc and n-BuOH extracts revealed the presence of important known antioxidant compounds such as ellagic acid derivatives, arjunolic acid, betulinic acid and β-sitosterol. The anti-inflammatory properties of D. thollonii extracts were investigated using RAW 264.7 murine macrophage cells. The MeOH extract reduced the stimulated NO production in a concentration-dependent manner. 86% reduction was observed at the highest tested concentration of 100μg/ml (IC50=5.9μg/ml). The n-BuOH extract showed higher dose dependent reduction of NO formation (IC50=6.5μg/ml) than the EtOAc extract (IC50=18.1μg/ml), whereas the water extract had no significant influence on the NO production. All the extracts did not have any influence on the macrophage viability. The phytochemical investigation of the EtOAc and n-BuOH extracts revealed that the main compounds identified do have potent anti-inflammatory properties. ConclusionThe biological and phytochemical characterization of the root extracts of D. thollonii validates the use of this plant for the treatment of inflammation and sinusitis, thus providing evidence that this plant extracts, as well as some of the isolated compounds, might be potential sources of antioxidant and anti-inflammatory drugs.