Abstract
The peels essential oil profiles of four new cultivars of Citrus species have been studied by GC-MS. The hydro-distilled oils yield was 0.1 - 1.0% (w/w) with the highest yield in lime peel. Out of the forty-one characterized components, limonene was the major monoterpene hydrocarbons detected in all tested samples (89.3 ± 0.0, 88.5 ± 0.2, 78.1 ± 0.3 and 53.0 ± 0.0% in grapefruit, orange, mandarin and lime oils, respectively). Both β-pinene (16.0 ± 0.5%) and p-cymene (13.9 ± 0.2%) were major in lime oil, while mandarin oil was rich with γ-terpinene (9.0 ± 0.5%). Concentration of volatile oils constituents was calculated based on the average of 3 determination ± standard deviation (S.D.). Three citrus oils; orange (100 μl/ml), lime and grapefruit (250 μl/ml), demonstrate relatively equivalent antiradical potential (inhibit 70% of DPPH) as vitamin E (5.2 μl/ml), having IC50 of 110 ± 10.0, 142 ± 2.5 and 155 ± 5.0 μl/ml, respectively. Lime peel essential oil showed the highest antimicrobial activity recording MIC90 of 14, 11, 12 and 9 μl/ml against Staphylococcus aureus, E. coli, Candida albicans and Aspergillus flavus, respectively, while other tested oils demonstrated moderate antimicrobial activity against the eight pathogenic microorganisms.
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