Abstract
The pathogenicity of the bacteria is increasing at an alarming rate as they have developed several means to overcome the treatments done for their removal. The Biofilm formation is one of that major ways used by the bacteria to increase its sustainability. This biofilm provides rigidity and resistance to the bacteria against the antibiotics. There is a requirement of another approach to treat such pathogen forming this rigid barrier. Nanoparticles have proven to be effective against such type of barrier and have shown to be potent anti bio film agents. The present study aims on developing the selenium nanoparticles using the biological and chemical methods to act against the bio film forming bacteria that is S. aureus and P. aeruginosa. The biofilm forming bacteria were isolated from the sewage water sample on the selective media and further characterized through staining and biochemical test. The selenium nanoparticle was synthesized biologically using Emblica officinalis extract and chemically using ascorbic acid along with sodium selenite in both the cases. The synthesized nanoparticle was characterized by UV-visible spectra and its antibacterial activity was checked through ‘Agar well diffusion’ against the isolated bacteria. The antioxidant property of both nanoparticles was analyzed through FRAP assay.
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