Abstract
The antimicrobial activity of three varieties of Vigna unguiculata L. Walp seed oil (LBS-1, LBS-2 and LBS-3) were investigated against five Gram positive bacteria ( Bacillus megaterium, Bacillus subtilis, Sarcina lutea, Salmonella typhi and Staphylococcus aureus ) and four Gram negative ( Escherichia coli, Shigella dysenteriae, Shigella sonnei, Shigella shiga ) and four fungi ( Penicilium spp., Mucor spp., Candida albicans and Aspergillus fumigatius ). The LBS-1 oil at the concentration of 400 I¼g/ disc showed the highest activity against Sarcina lutea (19±0.1 mm) than that of LBS-2 (14±0.3 mm) and LBS-3 (12±0.3 mm) oil whereas LBS-3 oil showed highest activity against Staphylococcus aureus (16±0.1 mm) than that of LBS-1 (10±0.6 mm) and LBS-2 (13±0.4 mm) oil. All the three oils are active against the three tested fungi namely Penicilium spp., Mucor spp. and Candida albicans but showed no sensitivity against Aspergillus fumigatius .
Highlights
In the treatment of infectious diseases, microorganisms have developed resistance to many antibiotics [1]
The antibacterial activity of LBS-1 oil, LBS-2 oil and LBS-3 oil were tested against nine bacteria at concentrations of 200 μg/disc and 400 μg/disc
The results of this study showed that the LBS-1, LBS-2 and LBS-3 oil have varied antimicrobial activities against the tested microorganisms
Summary
In the treatment of infectious diseases, microorganisms have developed resistance to many antibiotics [1]. By a number of studies worldwide, the antimicrobial properties of plants have been investigated and because of their antimicrobial properties, many of them have been used as therapeutic alternatives [3]. The plants such as Orthosiphon stamineus Benth [4], Coccinia grandis L. By mixing seed powder with oil, it is used as medicine of stubborn boils [11]. Boiled seeds are eaten to destroy worms in the stomach [12]. Walp seeds [14], Extraction and Characterization of Vigna unguiculata L. Walp seed oil [15] and Antidiabetic
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