Abstract
Objective:To assess the in vitro antibacterial, antifungal and cytotoxic activities of amblyone, a triterpenoid isolated from Amorphophallus campanulatus (Roxb).Methods:Disc diffusion technique was used for in vitro antibacterial and antifungal screening. Cytotoxicity was determined against brine shrimp nauplii. In addition, minimum inhibitory concentration (MIC) was determined using serial dilution technique to determine the antibacterial potency.Results:Large zones of inhibition were observed in disc diffusion antibacterial screening against four Gram-positive bacteria (Bacillus subtilis, Bacillus megaterium, Staphylococcus aureus and Streptococcus pyogenes) and six Gram-negative bacteria (Escherichia coli, Shigella dysenteriae, Shigella sonnei, Shigella flexneri, Pseudomonas aeruginosa and Salmonella typhi). The MIC values against these bacteria ranged from 8 to 64 μg/ml. In antifungal screening, the compound showed small zones of inhibition against Aspergillus flavus, Aspergillus niger and Rhizopus aryzae. Candida albicans was resistant against the compound. In the cytotoxicity determination, LC50 of the compound against brine shrimp nauplii was 13.25 μg/ml.Conclusions:These results suggest that the compound has good antibacterial activity against the tested bacteria, moderate cytotoxicity against brine shrimp nauplii and insignificant antifungal activity against the tested fungi.
Highlights
The frequency of life-threatening infections caused by pathogenic microorganisms has increased worldwide and is becoming an important cause of morbidity and mortality in immunocompromised patients in developing countries.[1] a large number of antimicrobial agents have been discovered, pathogenic microorganisms are constantly developing resistance to these agents.[1]
Candida albicans was resistant against the compound
LC50 of the compound against brine shrimp nauplii was 13.25 μg/ml. These results suggest that the compound has good antibacterial activity against the tested bacteria, moderate cytotoxicity against brine shrimp nauplii and insignificant antifungal activity against the tested fungi
Summary
The frequency of life-threatening infections caused by pathogenic microorganisms has increased worldwide and is becoming an important cause of morbidity and mortality in immunocompromised patients in developing countries.[1]. A large number of antimicrobial agents have been discovered, pathogenic microorganisms are constantly developing resistance to these agents.[1] In recent years, attempts have been made to investigate the indigenous drugs against infectious diseases.[2] This may help to develop safer antimicrobial drugs.[2]. [4,5] Previously, we have reported the possible antibacterial, antifungal and cytotoxic activities of tuberous roots of Amorphophallus campanulatus.[24] The present study was conducted to determine the antibacterial, antifungal and cytotoxic activities of amblyone, a triterpenoid isolated from tuberous roots of the plant (Fig 1)
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