Abstract
In this study select two active isolate from nine isolates of local agricultural soil of Mosul, were collected from 10 sample of 6 different region depending on antimicrobial activity to some test bacteria and fungi, Identified as gram-positive rod-shaped bacteria and, biochemical test, the selected isolates belonged to Bacillus genus, gave signal B1 and B2 were effective against both pathogenic bacteria Gram-positive and Gram-negative, two isolates gave high inhibition zone about 23mm against Staphylococcus aureus whereas gave less inhibition zone against Escherichia .coli, Klebsiella spp, Aspergillus niger and Candida spp, 20, 16, Furthermore studied the effect of temperature on the growth of two isolates which gave good growth at 40-45 degree whereas there was no growth at low temperature 5°C. Furthermore studied the effect of salt on the growth of two isolates that gave good growth at concentration 3% NaCl; the two strains were analysed utilizing agar publishing way and cross streak method.
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