Antimicrobial Activity Of Silver Nanoparticles Synthesized with Extract of Tomato plant Against Bacterial and Fungal Pathogens

Abstract

Objective: Silver nanoparticles (AgNPs) have a wide range of applications. Environmental-friendly synthesis methods for these nanoparticles are more preferable due to their various advantages. This study aimed to synthesize AgNPs using the extract of the tomato plant in an easy and economical way. and testing this AgNPs against some human pathogens.Methods: Silver nanoparticles were synthesized using aqueous silver nitrate and reducing tomato plant extract. The characterization of AgNPs was determined by ultraviolet-visible spectrophotometry (UV-Vis), X-ray crystallography (XRD) Scanning electron microscopy (SEM), Fourier transform infrared Spectroscopy (FT-IR), energy dispersive X-ray spectrum (EDAX), thermogravimetric - differential thermal analysis (TGA-DTA) data. The effects of the particles on pathogenic microorganisms were determined by minimum inhibition concentration (MIC).Results: These data, with a maximum absorbance of 450.51 nm, in the spherical view, with the peaks and values of 111o, 200o, 220o and 311o (38.08, 44.28, 64.42 and 77.34), AgNPs showed a cubic crystal structure and, using the Debye-Scherrer equation, it was determined that they had a crystal size of 21.11 nm AgNPs had an antimicrobial activity on hospital pathogens gram negative, gram positive and Candida albicans yeast. Conclusion: We found that these particles showed antimicrobial activity on various microorganisms even at very high concentrations. As a solution to the antimicrobial search, it can be developed in medical industry.