Abstract
Bacterial resistance is the mechanism through which the bacteria can decrease the action of antimicrobial agents. Studies have reported an antimicrobial action of Luma apiculata, however, there are no in vitro studies demonstrating the effect of this plant against multidrug-resistant bacteria. The objective of this study was to explore the antimicrobial properties of alcoholic extract of Luma apiculata against standard multi-resistant bacterial pathogens. Multidrug-resistant strains of Staphylococcus aureus ATCC 43300 (MRSA) and Klebsiella pneumoniae ATCC BAA 1705 (CRKP) were incubated independently for 72 h at 37oC with different concentrations (1-10,000 µg/mL) of alcoholic extract of Luma apiculata. The growth curve was studied up to 72 hours. The minimum inhibitory concentration (MIC) was determined by broth macrodilution method. The agar diffusion method was used to examine bacteriostatic activity and bactericidal activity was evaluated by a commercial kit and analyzed by flow cytometry. Analyses of chromatographic data, as well as mass spectrometry, were performed to determine the main compounds present in the extracts. Luma apiculata leaves extracts showed bactericidal activity and was effective attenuating growth in both bacterial strains. Thus, Luma apiculata could represent a natural alternative against multidrug-resistant bacterial strains. However, further studies should be undertaken to test their clinical utility. Keywords: Antimicrobial activity; Arrayan; Flavonoids; Medicinal plants; Multiresistant bacteria http://dx.doi.org/10.19045/bspab.2019.80140
Highlights
Bacterial resistance is the mechanism through which the bacteria can decrease the action of antimicrobial agents
The objective of this study was to explore the antimicrobial properties of alcoholic extracts of Luma apiculata leaves against standard multiresistant bacterial pathogens
Flavonoids are soluble in water, better recovery is achieved by using polar solvents such as ethanol, obtaining a greater amount of phenolic and flavonoid compounds which are attributed with antibacterial activity [24]
Summary
Bacterial resistance is the mechanism through which the bacteria can decrease the action of antimicrobial agents. Studies have reported an antimicrobial action of Luma apiculata, there are no in vitro studies demonstrating the effect of this plant against multidrug-resistant bacteria. The objective of this study was to explore the antimicrobial properties of alcoholic extract of Luma apiculata against standard multi-resistant bacterial pathogens. (CRKP) were incubated independently for 72 h at 37oC with different concentrations (1-10,000 μg/mL) of alcoholic extract of Luma apiculata. Luma apiculata leaves extracts showed bactericidal activity and was effective attenuating growth in both bacterial strains. Luma apiculata could represent a natural alternative against multidrug-resistant bacterial strains. Antimicrobial resistance (AR) represents an different hosts and rapidly spreads between important health issue that requires continents [1, 2]. The emergence of antibiotic-resistant forms of pathogenic S. aureus is a worldwide problem in clinical medicine [4]
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