Abstract
Objective: The aim of this study was to evaluate the antimicrobial effectiveness of calcium hydroxide associated with a two conventional (anesthetic liquid and olive oil) and a new vehicle (Triethanolamine). Material and methods: Initially, microorganisms were collected from four upper incisors presenting unsatisfactory root canal treatments and persistent apical periodontitis from the same patient using sterile absorbent paper cones placed inside the root canal following initial access and root filling removal. Next, they were grown in a BHI culture medium for 24 h. Afterwards, they were placed in 10 Petri dishes with 3 holes, where different formulations of the medications studied were placed. After 48 and 72 h, readings were taken of the growth inhibition halos. Results: After using Kruskal-Wallis test with significance level of 1% (? = 0.01) it was observed that, calcium hydroxide associated with Triethanolamine, produced results similar to those produced by the anesthetic liquid. Both combinations were superior to the association with olive oil. Conclusion: Considering the results of this in vitro study, it is possible to consider that Triethanolamine can be a viable alternative to be used as a vehicle associated with the calcium hydroxide.Keywords: Vehicles; Calcium hydroxide; Endodontics.
Highlights
Calcium hydroxide pastes are the most widely used intracanal medications for infectious processes involving primarily the pulp tissue and subsequently the periradicular tissues [1,2,3,4]
Considering the importance of pH for the antimicrobial effects of calcium hydroxide pastes associated with different vehicles, the aim of this study was to evaluate the antimicrobial effects of calcium hydroxide associated with Triethanolamine, anesthetic liquid and olive oil
Afterwards, fresh cultures of these microorganisms were cultivated in 5ml of brain heart infusion (BHI) broth culture medium, incubated at 37 °C for 72 h under aerobic conditions [18]
Summary
Calcium hydroxide pastes are the most widely used intracanal medications for infectious processes involving primarily the pulp tissue and subsequently the periradicular tissues [1,2,3,4]. In regard to the mechanism of action of these pastes, hydroxyl ion diffusion alters the pH of the medium and compromises the enzymatic metabolism of bacteria, causing protein denaturation and irreversible damage to the cytoplasmic membrane [5,6]. With this in mind, many substances have been researched to act as vehicles associated with calcium hydroxide [7,8,9]. Triethanolamine is clear, totally water soluble and miscible with most liquid oxygen organic solvents It is used for different purposes in the pharmaceutical field, such as a surfactant and emulsifier in manufacturing soaps [12]. Considering the importance of pH for the antimicrobial effects of calcium hydroxide pastes associated with different vehicles, the aim of this study was to evaluate the antimicrobial effects of calcium hydroxide associated with Triethanolamine, anesthetic liquid and olive oil
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