Abstract
The goal of endodontic treatment is to prevent and control of pulp and periradicular infections. Calcium hydroxide has a beneficial biological property as an intracanal medicament and can be combined with cresotin to disinfect bacteria in root canals, especially Enterococcus faecalis (E. faecalis) which is the most frequently isolated strain in the root canals. The aim of this study was to investigate in vitro the antimicrobial activity of calcium hydroxide, cresotin, and combination calcium hydroxide and cresotin (Ca[OH]2+Cresotin, 1:1 and 1:2) against E. faecalis. Antibacterial activity was determined by the agar diffusion method. The test medicaments were placed inside the hole that made in the inoculated agar medium. The zone of growth inhibition was measured and recorded after incubation for each plate, and the result was analysed statistically with ANOVA. The in vitro antimicrobial effects of combination calcium hydroxide and cresotin (Ca[OH]2+Cresotin, 1:2) has more prominent antimicrobial activity than others, and calcium hydroxide is more effective than cresotin alone. The antimicrobial activity of combined calcium hydroxide and cresotin is more effective in killing E. faecalis in comparison to the other treatments.
Highlights
Root canal infection is the result of colonisation of microorganisms, mainly dominated by anaerobic bacteria (Jaju & Jaju, 2011)
One of these bacteria is Enterococcus faecalis (E. faecalis) which is often isolated from root canal treatment cases
The sample used in this study was the bacteria E. faecalis suspension which was inoculated to brain heart infusion (BHI) agar media
Summary
Root canal infection is the result of colonisation of microorganisms, mainly dominated by anaerobic bacteria (Jaju & Jaju, 2011). One of these bacteria is Enterococcus faecalis (E. faecalis) which is often isolated from root canal treatment cases. E. faecalis is a normal flora in the oral cavity, and this bacterium is often found 4% to 40% in primary endodontic infections (Mulyawati, 2011). The bacteria have the ability to survive in the unsupportive and malnourished environment, involves in the invasion of the dentinal tubules, suppress the action on lymphocytes, forms biofilms and resistant to calcium hydroxide (Athanassiadis et al, 2007; Fisher & Phillips, 2009).
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
