Antimicrobial activity, minimum inhibitory concentration and cytotoxicity of thiadiazol compound

Abstract

Aim. This study aims to investigate the biological activity and cytotoxicity of the prepared thiadiazole derivative compound. Methods. A thiadiazol chemical, 4-[5-amino 1,3,4-thiadiazole-2-yl] phenol, was prepared by reacting 2.762 grams (0.01 moles) of 4-hydroxybenzoic acid with 1.822 grams (0.01 moles) of thiosemicarbazide. The prepared thiadiazole antimicrobial activity was tested at concentrations of 30, 50, 80, and 100 mg/mL against Escherichia coli, Pseudomonas, Bacillus cereus, and Staphylococcus epidermidis. Results. The thiadiazole chemical compound was prepared and tested against several bacterial species. The minimum inhibitory concentration (MIC) for Escherichia coli, Bacillus cereus, and Staphylococcus epidermidis was 0.8 mg/mL. Pseudomonas was unaffected by this substance. Investigations were made into the cytotoxicity activities. When the concentration was less than 0.01 mg/mL, it was discovered that the produced thiadiazol also had no impact on the red blood cell. Conclusion. The results revealed that the thiadiazole chemical compound had strong antibacterial activity against some of the pathogenic bacteria. This means that the chemical compound can be used as a narrow spectrum antibiotic.