Abstract
Ethanolic extract of Plumeria acutifolia Poir. (Apocynaceae) stembark was tested for antimicrobial activity against Gram-positive bacteria (Bacillus subtilis, Enterococcus faecalis, Staphylococcus aureus), Gram-negative bacteria (Escherichia coli, Klebsiella Pnumoniae, Psuedomonas aeruginosa, Salmonella typhimurium) and fungi (Aspergillus niger and candida albicans) by disc diffusion method. Minimal inhibitory concentration (MIC) and acute toxicity were also assayed. The ethanol extract showed the strong in vitro antimicrobial activity against E. faecalis, B. subtilis, S. aureus, P. aeruginosa, S. typhimurium, A. niger and C. albicans. The extract did not show any toxic symptoms against the tested mice. Key words: Plumeria acutifolia Poir, antibacterial activity, antifungal activity, disc diffusion, M.I.C, M.B.C/M.F.C, acute toxicity.
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