Abstract
The present work was aimed to illustrate how the use of Diode laser or silver nanoparticles (AgNPs) each of them individually, as an antibacterial agent against Staphylococcus aureus (S. aureus) as well as study the effective result from the absorption of laser energy by these nanoparticles to kill or inhibition bacterial growth. The silver nanoparticles are prepared by biological method. S. aureus are isolated and identified in the Central Health Laboratory in Al Najaf city. Nanoparticles are tested against S. aureus cultured on Muller Hinton agar but Diode laser or laser with nanoparticles are tested against S. aureus cultured in Nutrient broth. S. aureus are irradiating by Diode laser with different irradiation times, moreover there are different concentrations of AgNPs have been employed for killing and inhibition bacterial growth. Results showed that S. aureus is affected by AgNPs (mainly highest concentration of nanoparticles) also detected that decrease bacterial availability with increase time of irradiation to laser.
Highlights
2.Materials and MethodsDrug resistance by pathogenic bacteria such as S. aureus remains worldwide problem [1]
2.6.Application Laser Irradiations with Nanoparticles on Bacterial Species: The procedure for applying the Diode laser on the bacterial samples includes take tubes that contain S. aureus bacteria cultured in Nutrient broth with different concentrations of AgNPs and irradiate them with Diode laser with different irradiation times (1,3,5,7 and 10 min)
The size and morphology of nanoparticles were determined by UV-Vis spectroscopy in College of medicine - University of AlQadisiyah and scanning electron microscope (SEM) in College of Science, University of Al-Kufa as seen in fig.1 and fig. 2
Summary
Drug resistance by pathogenic bacteria such as S. aureus remains worldwide problem [1]. The aim of the study: The present study was aimed to identify the antibacterial effect of Diode laser and silver nanoparticles (alone or in combination with each other) on one of pathogenic bacteria (S. aureus). 2.5.Application Laser Irradiations on S. aureus: The procedure for applying the Diode laser (with 50 mW) on the bacterial samples includes take tubes that contain S. aureus cultured in Nutrient broth and irradiate them with Diode laser with different exposure times (1,3,5,7,10,15 and 20 min). 2.6.Application Laser Irradiations with Nanoparticles on Bacterial Species: The procedure for applying the Diode laser (with 50 mW) on the bacterial samples includes take tubes that contain S. aureus bacteria cultured in Nutrient broth with different concentrations of AgNPs and irradiate them with Diode laser with different irradiation times (1,3,5,7 and 10 min)
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: International Journal of Engineering & Technology
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
