Antimicrobial Effect of Diode Laser and Biosynthesis Silver Nanoparticles on Escherichia Coli in Vitro

Abstract

The laser and nanoparticles representing the concourse of biological science with physical science and considering modern method that used as an antibacterial instead of conventional antibiotics. The present study is an attempt to illustrate how the use of laser or silver nanoparticles each of them individually, as an antibacterial agent against Escherichia coli (E. coli) as well as study the effective result from theabsorption of Diode laser energy by these nanoparticles to kill or inhibition bacterial growth. The silver nanoparticles (AgNPs) are prepared by biological method E. coli were isolated and identified by use MacConkey agar and biochemical tests. Nanoparticles are tested against E. coli cultured on Muller Hinton agar but Diode laser or laser with nanoparticles are tested against E. coli that cultured in Nutrient broth. E. coli are irradiating by Diode laser with different times. Different concentrations of silver nanoparticles have been employed for killing and inhibition bacterial growth. Results showed E. coli was effected by nanoparticles (mainly highest concentration of nanoparticles) also detected that decrease bacterial availability with increase time of exposure to Diode laser