Abstract
Astragaloside IV (ATS)is an active component of Astragalus membranaceus,which has immune regulation and anti-inflammatory effects. However, owing to its large molecular weightand poor solubility in water, the therapeutic effects of ATSare limited.We aimed to prepare astragaloside IV-chitosan (ATS-CS) nanoparticles and determinetheir anti-inflammatory effect and mechanismof actionon RAW264.7 cells usingmetabolomics. The size of the ATS-CS nanoparticleswas 200.3 nm with a zeta potential of 30.5mV, and the encapsulation rate and drug loading were 69% and 13%, respectively. ATS-CS nanoparticlesnot only significantlydecreasedthe increase of NO, IL-6 and TNF-αlevels induced by LPS, but also exerted an anti-inflammatory effect by acting on arginine and proline, glutathione, sphingolipid, glycerophospholipid, and glycine, serine,and threonine metabolism. Our findings confirmedthat ATS-CS nanoparticleshave good anti-inflammatoryactivity and showed that the activity of high molecular weightcan be increased byproducingnanoparticles.Our study paves the way for exploring the mechanism of nanoparticles through metabolomics.
Published Version
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call