Anti-inflammatory and anti-arthritic activity in extract from the leaves of Eriobotrya japonica.

Abstract

The Eriobotrya japonica (EJ) is a Chinese medicinal plant that is currently grown in Brazil. E. japonica leaves infusion is traditionally used in the treatment of inflammation; however, there are few scientific studies showing the effects of these properties on joint articular and persistent experimental inflammation. The present research had objective investigation of the effect of infusion obtained from leaves of E. japonica (EJLE) on acute and persistent experimental articular inflammation. The Swiss mice were treated orally with EJLE and analyzed for acute pleural inflammation (30, 100, and 300mg/kg), paw edema induced by carrageenan (100mg/kg), acute knee inflammation induced by zymosan (100mg/kg), and persistent inflammation induced by Complete Freund's Adjuvant (CFA) (30 and 100mg/kg). Mechanical hyperalgesia, cold and edema were analyzed. The chromatographic analysis of EJLE revealed the presence of corosolic acid, oleanolic acid, and ursolic acid. EJLE presented anti-inflammatory activity in the pleurisy model, inhibiting leukocyte migration, protein extravasation and nitric oxide production. In the articular inflammation model, EJLE reduced the number of leukocytes in the joint cavity, paw edema and hyperalgesia (4h after induction). In the persistent inflammation model induced by CFA, the extract reduced paw edema after 11 days of mechanical and cold hyperalgesia on day 6. The EJLE has anti-inflammatory and antihyperalgesic potential in models of acute and persistent experimental articular inflammation, making this infusion a new possibility for complementary treating acute or chronic articular inflammatory diseases.