Abstract
Purpose: To examine the effects of A. blazei Murill on the production of proinflammatory cytokine TNF-α and regulatory cytokine IL-10 in the spleen of mice fed a high-fat diet.Methods: The study was conducted on 25 BALB/c male mice divided into five groups consisting of five mice in each group and utilized three A. blazei Murill extract doses: 100, 200 and 400 mg/kg. The mice were fed a high-fat diet (HFD) and given A. blazei Murill extract for 12 weeks. The relative number of CD4+TNF-α+, CD11b/c+TNF-α+, and CD4+CD25+IL-10+ were measured using flow cytometry.Results: Oral administration of A. blazei Murill extract (100 mg/kg) in mice fed a high-fat diet significantly decreased (p < 0.05) the level of TNF-α that produced by CD4+ T cells and macrophages (1.64 %), compared with control. The 200 mg/kg dose decreased the level of CD11b/c+TNF-α+ by 5.37 % (p < 0.05) compared to 100 mg/kg dose. The 400 mg/kg dose significantly enhanced regulatory cytokines IL-10 by 17.56 % (p < 0.05) in mice fed a high-fat diet.Conclusion: This findings suggest that Agaricus blazei Murill can inhibits processes in HFD-induced mice by reducing TNF-α production and increasing the anti-inflammatory cytokine IL-10. These results provide new insight into the pharmacological actions of Agaricus blazei Murill as a medicinal food for potential therapy of atherosclerosis disease.Keywords: Anti-inflammatory agent, Agaricus blazei Murill, Proinflammatory cytokine, TNF-α, IL-10
Highlights
Atherosclerosis is one of the factors that causes mortality arising from cardiovascular disease [1,2]
Effect of A. blazei on the level of Tumor Necrosis Factor-α (TNF-α) production by CD4+ T cells relative number of this cytokines significantly compared to the normal group
In D3 treatment of A. blazei Murill extract, the number of TNF-α was similar to the normal mice
Summary
Atherosclerosis is one of the factors that causes mortality arising from cardiovascular disease [1,2]. In the later stages of atherosclerosis, vascular remodeling is accelerated by TNFα; wall thickness of the ascending aortic arch is increased at 11 weeks of age in ApoE KO mice, but only at 26 weeks in TNFα/ApoE double KO mice [12] Mice deficient for both TNFα and ApoE exhibit a reduction of atherosclerotic lesion size [13,14]. We investigated the anti-inflammatory effect of A. blazei Murill extract in mice fed a high-fat diet. A. blazei Murill extract was administered in mice by oral gavage once a day for 12 weeks. Random experiments were conducted with 5 replications in 5 groups: the control group, the high fat diet group, the high fat diet group followed by Agaricus blazei murill extracts administration at 3 different doses: 100, 200, and 400 mg/kg. All mice were first fed with standard diets for 1 week for acclimatization before the study. Significant treatment effect was further analyzed for between-treatment differences with Tukey HSD Test
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