Antihypertension Effect from Bakasang’s Peptide Extract Based on MDA Levels in Sera and iNOS Expression in Cardiac Tissue of Rats Hypertensive Model

Abstract

Hypertension is an abnormality of cardiovascular condition, marked by high-blood pressure over normal condition. One of the factors that causes hypertension is altered equilibrium of Renin-Angiotensin-Aldosteron system, which commonly caused by genetic disorder, could produces excess of Angiotensin II which known as vasoconstrictor. Angiotensin II elevates body’s blood pressure and stimulates production of free radicals causes hypertension and oxidative stress. This research conducted to explore potential antihypertension effect from bakasang’s peptide extract made from fermented skipjack tuna (Katsuwonus pelamis L.) based on MDA levels in sera and iNOS expression in cardiac tissue from hypertension rats (Rattus norvegicus) induced by DOCA-salt. Rats were divided into five groups, they were: (1) negative control group, (2) hypertensive control group, (3) hypertension with captopril therapy with dose of 5 mg/kg of body weight (BW), (4,5) bakasang’s peptide extract therapy with dose of 200 mg/kgBW, and 300 mg/kgBW respectively. The results showed that bakasang’s peptide extract with dose of 200 mg/kgBW significantly decrease (p less than 0.05) MDA level in sera and iNOS expression in cardiac tissue. MDA level decreased up to 32.77%, and iNOS level of expression decreased up to 34.46 % after therapy using bakasang’s peptide extract on hypertension rats. It can be concluded that bakasang’s peptide extract has antihypertension effect (ACE-inhibitor), and capable to decrease MDA levels in sera and iNOS expression in cardiac tissue on hypertension rats induced by DOCA-salt.