Abstract
Type 1 diabetes (T1D) poses a significant global health challenge, with current treatments often proving inadequate. This study provides a comprehensive evaluation of the antihyperglycemic effects of methanolic extract from Algerian Trigonella foenum-graecum L. seeds (MEF) in type 1 diabetic rats, combining both in-vivo and in-silico studies: molecular docking and ADMET. Polyphenol compounds (PCs) identified. Twenty-four male rats divided into four groups: control, normal anddiabetic rats treated with MEF. T1D was induced via a single 50 mg/kg streptozotocin injection. MEF was administered orally at a dose of 400 mg/kg b.w. for 28 days, with measurements of body weight and fasting blood glucose. Histopathological examinations of pancreatic tissues were conducted post-treatment. HPLC identified fifteen PCs. We found that six main PCs had strong binding affinities for the IR (-9.463 to -7.893 kcal/mol). The ADMET modeling indicated favorable drug-likeness, oral bioavailability, and no toxicity risk. Significantly, diabetic rats treated with MEF exhibited a marked reduction in fasting blood glucose levels (494.67 mg/dl to 144.17 mg/dl; p < 0.001), along with an increase in body weight and improvements in the pancreatic islets of Langerhans. These findings suggest that MEF possesses substantial antihyperglycemic potential and may play a role in managing T1D complications.
Published Version
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