Abstract
Insulin leave (Tithonia diversifolia (Hemsl.) A. Gray) empirically used by people as antihyperglycemic drugs. The study was aimed to evaluate antihyperglycemic activity as well as to determine the most optimum dose of T. diversifolia to reduce blood glucose levels. Hyperglycemic condition was induced to male Rattus norvegicus rats by intraperitoneal injection of alloxan at a dose of 150 mg/kg bw (body weight). In this study, completely randomized design was performed with three treatment groups and five times repetition. Group P1 received glibenclamide 10 mg/kg bw, P2 received aqueous extract of T. diversifolia at a dose of 150 mg/kg bw, P3 received aqueous extract of T. diversifolia at a dose of 300 mg/kg bw. Administration of both glibenclamide and aqueous extract of T. diversifolia was conducted orally for 28 days. Data were analyzed using Anova and Duncan’s test with 95% confidence level (α = 0.05). The results showed that the mean percentage of decrease in blood glucose levels, drink intake and body weight of all treatment groups were not significantly different (p>0.05). Data of feed intake showed that P1 was significantly different from P2 (p<0.05), but P2 was not significantly different from P3 (p>0.05). Based on the result of this research, it was found that the low doses of aqueous extract of T. diversifolia has the same ability to decrease blood glucose level compared to glibenclamide. Futhermore, this study provide some information that can be used as preclinical analysis to determine effective doses of aqueous extract of T. diversifolia to decrease blood glucose levels.
Highlights
Diabetes mellitus (DM) is a disorder of the endocrine system in carbohydrate metabolism due to insulin secretion deficiency characterized by the occurrence of hyperglycemia
This study aimed to analyze the response of blood glucose levels, food and drink intake as well as the body weight of hyperglycemic rats after administration of aqueous extract of T. diversifolia leaves at doses of 150 mg/kg BW and 300 mg/kg BW for 28 days
The percentage in decreasing of blood glucose levels in the treatment aqueous extract of T.diversifolia both doses of 150 mg/kg BW/days and 300 mg/kg BW/days is lower than glibenclamide treatment, but they have no big differences
Summary
Diabetes mellitus (DM) is a disorder of the endocrine system in carbohydrate metabolism due to insulin secretion deficiency characterized by the occurrence of hyperglycemia. Various agents used to reduce blood glucose levels with different working mechanisms are available, but there are still obstacles to achieve the glycemic targets. Those obstacles include the increase in the risk of hypoglycemia, mortility and body weight (Hasan et al, 2010). Glibenclamide is a hypoglycemic drug from the sulfonylurea class working by stimulating pancreatic beta cells to secrete insulin (Rai et al, 2012). Alternative therapies that might be done are the using of herbal medicines, because people have relied on traditional medicines for health care since long time ago
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