Anti-human gastric cancer study of gold nanoparticles synthesized using Alhagi maurorum

Abstract

One of the main nanomaterials is gold nanoparticle. During current researches, it has been indicated that these nanoparticles have high cytotoxic activities against abnormal cancer cells. Gold nanoparticles are composed of silver surrounded by oxygen networks. This nanoparticle has the antioxidant behaviors including superoxidase dismutase, catalytic enzyme activity, nitric oxide and hydroxyl radical inhibition, as well as oxidant behaviors that determine several conditions such as the pH of the environment, the type of activity of this nanoparticle. In the present study, gold nanoparticles were green-synthesized using the aqueous extract of Alhagi maurorum leaf aqueous extract. The synthesized AuNPs were characterized by analytical techniques including TEM, XRD, EDX, FE-SEM and UV-Vis., and FT-IR. The nanoparticles were formed in a spherical shape in the average size of 18.36 nm. In the oncological part of the recent study, the treated cells with AuNPs were assessed by MTT assay for 48h about the cytotoxicity and anti-gastric carcinoma properties on normal (HUVEC) and gastric carcinoma cell lines i.e. GC1415, GC1436, NCI-N87 and MKN45. The viability of gastric carcinoma cell lines reduced dose-dependently in the presence of AuNPs. The IC50 of AuNPs were 44, 125, 45, and 22 µg/mL against GC1415, GC1436, NCI-N87 and MKN45 cell lines, respectively. In the antioxidant test, the IC50 of AuNPs and BHT against DPPH free radicals were 48 and 37 µg/mL, respectively.