Antigenotoxic, antimutagenic and cytoprotective potential of Salvia hispanica L. seed extract on human leukocytes exposed to oxidative damage

Abstract

This study assesses the antigenotoxic activity, antimutagenic and inhibiting cytotoxic conferred for the extract of seeds of Salvia hispanica L. (ShE) in culture of human leukocytes. Phytochemical methods were used by HPLC for determination of compounds: chlorogenic acid, caffeic acid, gallic acid, kaempferol, quercetin and rutin. Cellular cultures were placed in contact with the extract at concentrations of 100µg/mL and 10µg/mL in addition to hydrogen peroxide as causative agent of damage. The results showed a reversal rate of DNA damage of 31%, 41.4% in the frequency of micronuclei, 21.7% in cytoplasmic rates of chromosomal abnormalities and a 17.7% improvement in cell viability in the sample containing 100µg/mL of ShE. The samples that contained the ShE in low concentration of 10µg/mL did not show such reversals against the effects of H2O2. We demonstrated that the use of ShE has beneficial and promising effects.