Abstract
Polyclonal antiserum was prepared against phospholipase A 2 from Naja naja and used to prepare a purified antibody. It cross-reacted with the antigen, and with intracellular mammalian PLA 2. This antibody was immunoreactive and inhibited the PLA 2 activity of Naja naja and of guinea pig alveolar macrophages or rat lymphocytes. By immunoblotting, this antiserum revealed one band of PLA 2 from Naja naja (14 kDa) and 3 bands for guinea pig alveolar macrophages and rat lymphocytes (30, 45 kDa and a minor band of 14 kDa). These results show an antigenic relatedness between an extracellular PLA 2 and membrane-bound PLA 2 from two different mammalian species and cell types.
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