Abstract
In vitro antigen-specific proliferation was investigated in a lymphocyte population that had been taken from the peritoneal exudate cells (PEC) of C3H/HeN mice (Iak) primed in vivo with both bacterial lipopolysaccharide (LPS) and horse red blood cells (HRBC) and had been purified by passage through a nylon fiber column (Nfc). The proliferative response of the Nfc-passed lymphocytes primed with HRBC and LPS [T(HRBC+LPS) cells] depended on the dose of antigen in the cultures, and the response was higher than that of cells prepared from mice primed with HRBC alone [T(HRBC) cells]. No response was seen in the cells prepared from the LPS-primed mice [T(LPS) cells] or normal mice [T(N) cells]. The response of the T(HRBC) cells was abolished by previous treatment of the cells with anti-Iak antibody and complement (C), whereas the response of the T(HRBC+LPS) cells was retained after the same treatment, indicating that the Ia- T(HRBC+LPS) cells can proliferate in response to antigen in spite of Ia+ accessory cell-depletion. Supernatants from the cultures of Ia- T(HRBC+LPS) cells in the presence of HRBC showed abundant IL-2 activity, while those of Ia- T(HRBC) cells did not. The IL-2 should be produced by the L3T4 cell population in T(HRBC+LPS) cells in response to antigen, since the previous treatment of the cells with anti-L3T4 antibody and C abrogated the production.(ABSTRACT TRUNCATED AT 250 WORDS)
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