Abstract
The integration of metallic or ceramic nanoparticles in polymer matrices has improved the antimicrobial and antifungal behavior, resulting in the search for composites with increased bactericidal and antimycotic properties. A polycaprolactone fibers with copper oxide nanoparticles was prepared. Polycaprolactone-copper fibers (PCL- CuONPs) were prepared into two major steps in situ method: (a) Synthesis of CuO particles, then (b) incorporation of polycaprolactone to electrospun process. The first step is the reduction of Cu+2 ions by gallic acid in N,N-dimethylformamide and tetrahydrofuran solution with the simple addition of polycaprolactone in the solution for the second electrospun step. Raman spectra provide information about the nature of the copper oxide synthesized. There are three Raman peaks in the sample, at 294 and 581 cm-1 and a very broad band from 400 to 600 cm-1 which are characteristics bands for CuO. Scanning electron microscopy (TEM) revealed copper oxide nanoparticles with semispherical shapes with diameter 35 ±11 nm. Dynamic light scattering (DLS) analysis showed uniform CuONPs in a range of 88±11 nm. Scanning electron microscopy (SEM) of PCL-CuONps reveled fibers with diameters ranging from 925 to 1080 nm were successfully obtained by electrospinning technique. Orientation, morphology and diameter were influenced by the increment on CuONPs concentration, with the smaller diameter present in samples prepared from low concentrated solutions. The antimycotic applicability of the composite was evaluated to determine the antifungal activity in three species of the genus Candida (Candida albicans, Candida glabrata and Candida tropicalis). PCL-CuONPs exhibit a considerable antifungal effect on all species tested. The preparation of PCL-CuONPs was simple, fast and low-cost for practical application as an antifungal dressing.
Highlights
The fast emergence of antibiotic and antifungal resistant bacteria and fungi is occurring on a world scale
We propose to solve such limitations is by merging PCL with CuONPs synthesized in a non-aqueous reducing solvent to prepare a composite with degradability, and controllable mechanical properties proportioned by the PCL and with antimicrobial activity against pathogenic Candida species depending on the initial concentration of CuONPs dispersed alongside the fibers
Our analysis shows the absorption peaks increasing to the precursor salt concentration from 270 to 285 nm
Summary
The fast emergence of antibiotic and antifungal resistant bacteria and fungi is occurring on a world scale. The most notable of the diseases present in the oral cavity in humans is a common fungal infection known as oral candidiasis. This is an opportunistic infection which may reflect immunological changes and a possible association with potentially malignant disorders of the oral mucosa. Nanomaterials based on metal ions, have an outstanding of cytotoxicity activity against bacteria, fungi and viruses Nanometallic materials display this antimicrobial property due to their surface change and surface to volume ratio, which allows an enhanced dilution of metal ions disabling enzymes and DNA of microorganisms creating an electron imbalance between donor groups such as thiol, carboxyl, and hydroxyl groups [8]. We propose to solve such limitations is by merging PCL with CuONPs synthesized in a non-aqueous reducing solvent to prepare a composite with degradability, and controllable mechanical properties proportioned by the PCL and with antimicrobial activity against pathogenic Candida species depending on the initial concentration of CuONPs dispersed alongside the fibers
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