Antifungal Resistance Profile for Clinical Isolates of Candida spp. at Institute Pasteur of Côte d’Ivoire

Abstract

Background: The aim of this study was to identify Candida species isolated from clinical samples and detect their susceptibility patterns to antifungal agents. Methodology: This study was conducted on clinical samples collected from patients with suspected candida infection referring to mycology laboratory of Institute Pasteur of Côte d’Ivoire for diagnosis. Candida species were isolated and identified using conventional and the innovative VITEK 2 microbial identification system. Antifungal susceptibility test for Amphotericin-B, 5-Flucytosine, Fluconazole, Itraconazole and Voriconazole were performed using ATB Fungus 3® of Biomerieux. Results: Most of the isolates were obtained from vaginal swabs 806 (64.8%), followed by Nail scrapings 49 (5.67%). Overall C. albicans counting was 58.9% (732/1234) of the infections. Sensitivity rates of C. albicans species from vaginal swab to 5-FC, AMB, FCZ, VCR and ITR were 97.26, 94.34, 94.89, 95.98 and 88.69 respectively. Resistance rates of C. albicans from vaginal swab were observed with AMB (5.66%) and ITR (5.29%). Sensitivity rate of C. glabrata was 100% with 5-FC, AMB and VCR. All C.krusei species were resistant to fluconazole. The sensitivity rate of C. tropicalis to the antifungal drugs tested varied between 91.66% with ITR to 100 with VCR. Conclusion: C. albicans was the most common of the candida species isolated in this study and remains sensitive to the drugs tested. The study showed resistance of all C. krusei strains to fluconazole. Knowledge about etiologic agents and their susceptibility patterns is helpful for successful treatment of the patients.