Abstract
In this study, eight endophytic fungi were isolated from the leaves, stems and roots of Michelia champaca. The isolates were screened and evaluated for their antifungal, anticancer and acetylcholinesterase (AChE) inhibitory activities. All of the extracts exhibited potent activity against two evaluated phytopathogenic fungi. Chemical investigation of EtOAc extracts of the endophytic fungus Colletotrichum gloeosporioides resulted in the isolation of one new compound, 2-phenylethyl 1H-indol-3-yl-acetate (1), and seven known compounds: uracil (2), cyclo-(S*-Pro-S*-Tyr) (3), cyclo-(S*-Pro-S*-Val) (4), 2(2-aminophenyl)acetic acid (5), 2(4-hydroxyphenyl)acetic acid (6), 4-hydroxy- benzamide (7) and 2(2-hydroxyphenyl)acetic acid (8). All of the compound structures were elucidated using 1D and 2D NMR and MS analyses. The antifungal and AChE inhibitory activities of compounds 1–8 were evaluated in vitro. Compound 1 exhibited promising activity against Cladosporium cladosporioides and C. sphaerospermum that was comparable to that of the positive control nystatin.
Highlights
The Colletotrichum genus is considered a major plant pathogen worldwide
Several new antimicrobial metabolites have been isolated from C. cladosporioides, including colletotric acid [2], the phytotoxin ferricrocin [3] and gloeosporone [4]
Three endophytic fungi were isolated from the stems (Mc-1, Mc-2, and Mc-3), four from the leaves (Mc-4, Mc-5, Mc-6, and Mc-7) and one from the roots (Mc-8) of M. champaca
Summary
The Colletotrichum genus is considered a major plant pathogen worldwide. The species. (teleomorph Glomerella cingulata) has been isolated as a plant pathogen [1] and an endophytic fungus [2] This species has been extensively investigated, for the production of secondary metabolites. Michelia champaca (Magnoliaceae) is native to Asia but is cultivated in Brazil This species is used in Brazilian traditional medicine for the treatment of various diseases, including rheumatism, inflammation, fever, cough and fertility regulation. This plant is reported to possess significant antitumor, anti-inflammatory, antimicrobial, antioxidant, antidiabetic, antidiuretic and memory enhancing activities [5]. The antifungal and anticholinesterase activities of these compounds were evaluated
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