Abstract
To investigate the antifungal activity of the compound preparation of traditional Chinese drug (KZY-2) on Candida albicans and Cryptococcus neoformans isolated from faecal specimen of pigeon in vitro. After KZY-2 effects on Candida albicans(ATCC32354, GFC1∼GFC10) and Cryptococcus neoformans(CCCMD2a, GFD1∼GFD10), the diameter of inhibited zone was measured adopting agar plate diffusion method and the MIC was measured Reference to the M27-A recommended by the United States National Laboratory Standards Committee (NCCLS). MFC were measured by colony counting method after agar plate culture and reference to the M27-A. The diameters of inhibited zone of KZY-2 against Candida albicans(ATCC32354) and Cryptococcus neoformans(CCCMD2a) were 28mm and 28mm, the MIC were 312.5μg/m1 and 156.3μg/m1, the MFC were 625μg/m1 and 312.5μg/m1. The diameter of inhibited zone of KZY-2 against Candida albicans(GFC1∼GFC10) which of seven were effective was between 12mm and 35 mm, the MIC was between 312.5μg/m1 and 2500μg/m1, the MFC were between 625μg/m1 and 5000μg/m1. The diameter of inhibited zone of KZY-2 against Cryptococcus neoformans(GFD1∼GFD10) which of nine were effective was between 18mm and 40mm, the MIC was between 156.3μg/m1 and 625μg/m1, the MFC were between 312.5μg/m1 and 1250μg/m1. These results show the diameters of inhibited zone and MIC were different when KZY-2 effects on different strain with the same drug concentration. Compared Candida albicans and Cryptococcus neoformans, the diameters of inhibited zone and MIC all had the significant difference, which the diameter of former was bigger, but the MIC of the latter was bigger. these results indicate The KZY-2 have good in vitro antifungal activity on Candida albicans and Cryptococcus neoformans whether which are the standard strains or isolated strains from faecal specimen of pigeon.
Published Version
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