Abstract
Background:The aim of the present study was to analyse the antidiabetic and antioxidant potential of GancidinW (GW) extracted fromStreptomyces paradoxusVITALK03.Materials and Methods:Antidiabetic potential of GW was evaluated by assay of α-amylase and α-glucosidase inhibitory activity; haemoglobin glycosylation and yeast glucose uptake. The antioxidant potential of GW was assessed by 2,2-Diphenyl-1-picrylhydrazyl (DPPH) assay, 2,2′-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical cation decolorization assay and superoxide assay. The inhibition of α-amylase and α-glucosidase by GW was also studied byin Silicomolecular docking analysis.Results:GW (1mg/ml) showed 69.32% of α-amylase and 54.04% of α-glucosidase inhibitory activity. GW (1mg/ml) prevented haemoglobin glycosylation up to 30.92% and the glucose uptake by yeast cells was increased up to 64.38%. The binding interaction GW with α-amylase showed the least free binding energy of -6.09Kcal/mol and -7.53Kcal/mol with α-glucosidase by docking studies. GW also demonstrated moderate antioxidant activity in all the antioxidant assays performed.Conclusion:The results of this study suggests that the antidiabetic and antioxidant potential of GW can be probed further to develop GW as effective antidiabetic agent.
Highlights
Actinomycetes are gram positive, saprophytic and filamentous bacteria belonging to the order Actinomycetales and the family Actinomycetaceae [1, 2]
Materials and Methods: Antidiabetic potential of GW was evaluated by assay of α-amylase and α-glucosidase inhibitory activity; haemoglobin glycosylation and yeast glucose uptake
The results of this study suggests that the antidiabetic and antioxidant potential of GW can be probed further to develop GW as effective antidiabetic agent
Summary
Actinomycetes are gram positive, saprophytic and filamentous bacteria belonging to the order Actinomycetales and the family Actinomycetaceae [1, 2]. Actinomycetes are one of the economically important bacteria and the genus Streptomyces are known for producing several bioactive compounds. Actinomycetes are prolific producers of numerous bioactive compounds and new chemical entities. Out of 23000 bioactive compounds isolated from the microorganisms, more than 10000 bioactive compounds were from actinomycetes, among them, 7600 were isolated from Streptomyces species [3]. Antibacterial, antifungal, antiviral, anticancer and antimalarial activities of bioactive compounds extracted from actinomycetes have already been reported [4]. In the recent years, the rediscovery of known bioactive compounds in terrestrial actinomycetes have increased and researchers have shown increased interest in exploring marine actinomycetes. The aim of the present study was to analyse the antidiabetic and antioxidant potential of GancidinW (GW) extracted from Streptomyces paradoxus VITALK03
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