Anticonvulsant Effects and Histopathological Changes in the Hippocampus of Pentylenetetrazole (PTZ)-induced Epileptic Mice Model following Mentat Treatment

Abstract

Abstract Background: Epilepsy is a neurological illness that disturbs the central nervous system and is characterized by regular convulsions. Over 70 million people worldwide are thought to have epilepsy, with the prevalence rate estimated to be around 1%. Aims: The objective of this study was to assess antiepileptic activities and histological changes after Mentat administration in the hippocampus of pentylenetetrazole (PTZ)-induced seizure mice. Materials and Methods: Twenty Swiss albino mice (18–28 g) were divided into four groups (n = 5) and were given the following intraperitoneally, 2 ml/kg distilled water and 50 mg/kg PTZ to Groups 1 and 2 animals, respectively. Groups 3 and 4 animals were given 200 mg/kg and 400 mg/kg of Mentat, respectively, 1 h before the administration of PTZ and were observed for 300 s. After the experiment, all surviving animals in the various groups were humanely sacrificed and the brains were harvested and preserved in 10% buffered formalin. The brain tissues were processed using routine histological procedures and stained with hematoxylin and eosin. Results: Results of this revealed that Mentat was able to delay the onset time of seizure and offered quantal protection to the animals. Mentat also showed a dose-dependent ameliorative effect against histological changes following PTZ administration in mice. Conclusion: Mentat attenuates PTZ-induced seizure in mice.