Anticoagulant Effects of Glycosaminoglycan Extracted from Fish Scales

Abstract

Background: Fisheries wastes are the unique sources of natural products which structural and chemical characteristics of their extracted compounds are different from those of terrestrial animals. They are known as a rich source of bioactive molecules, including collagen and glycosaminoglycan (GAGs).In the present study, we extracted and analysed anticoagulant activity of glycosaminoglycan from fish (Rutilus frisii kuum) scales Materials and Methods: The glycosaminoglycan compounds were extracted using cationic salt of cetylpyridinium chloride (CPC) and Fourier transform infrared spectroscopy (FTIR) spectrum was used to identify and compare the structure of the extracted glycosaminoglycan with heparin. Anticoagulant property of extracted glycosaminoglycan was measured by prothrombin time (PT), activated partial thromboplastin time (aPTT), and thrombin time (TT) on human blood at three concentrations of 50, 100, and 200 µg/ml. Results: The value of the extracted glycosaminoglycan was estimated approximately 23.8 mg/g of dry tissue.FTIR analysis results confirmed the presence of heparin-like compounds in fish scales glycosaminoglycan. Human Plasma coagulation time increased significantly with extracted GAGs concentration incriminated. Since at a concentration of 200 µg/ml, coagulation time as aPTT was 4.3 times that of control and coagulation time was prolonged about 138.6 seconds. Conclusions: The results of the current study showed that the glycosaminoglycan extracted from fish scales had valuable anticoagulant property compared to synthetic anticoagulant compounds such as heparin.