Anticoagulant Activity of Pineapple (Ananas comosus) Extract on Human Blood Samples

Abstract

The abundance, availability and affordability of pineapple have triggered the researcher to explore its potential for clinical laboratory applications. Thisstudy was conducted to determine the viability of pineapple (Ananas comosus)extract as laboratory anticoagulant and substitute for ethylenediamine tetraaceticacid (EDTA). The data obtained from laboratory standard procedures weresubjected to one-way analysis of variance with Tukey’s test. Among the fourmethods of extraction used, 95% ethanol and Expression gave a considerableyield with respect to volume of the extract produced. With regard to the ability toprevent blood coagulation, extract obtained through Expression with spiral twistobtained positive results comparable for EDTA. In the observation for red blood Vol. 6 · June 201453IAMURE International Journal of Science and Clinical Laboratorycell hemolysis, the different volumes of extracts produced results comparableto EDTA. However, with regard to the efficiency in preventing red blood cellcrenation and clumping, 300ul extract did not have a significant difference withEDTA; therefore, the results obtained were comparable. Preservation of thestaining reactions of the cell structures was also observed. Although significantdifferences were recorded in each of the volumes used, 300ul produced resultsthat were somehow comparable to EDTA. The efficiency was recorded in theevaluation of the extract’s efficiency in maintaining cell size and shape. Keywords: Medical Technology, anticoagulant property, pineapple extracts, calciumchelator, EDTA, bromelain, hemolysis, staining reaction, morphology, trueexperimental design,ANOVA, Tukey’s test, Philippines