Anti-cancer potential of the lipoidal and flavonoidal compounds from Pisum sativum and Vicia faba peels

Abstract

For a long time, plant secondary metabolites have been strongly examined for their antitumor and cytotoxic impacts. These days, there is another pattern of making utilization of the waste products of plants because of their extravagance of numerous phytochemical components and adequacy on human wellbeing. This research work is handling the effect of diversity of lipoidal and phenolic compounds found in the peels of two common edible plants in the Middle East; Pisum sativum and Vicia faba L. for their assesment as anticancer agents. The GC/MS of the n-hexane extract of both plant peels led to identification of twenty compounds (82.99%) and seventeen compounds (85.97%) of the total lipoidal contents from P. sativum and V. faba, respectively. While the HPLC analysis of the ethyl acetate fraction of the two plant peels resulted in recognition of 17 flavonoids and 18 phenolics from P. sativum and 16 flavonoids and 17 phenolics from V. faba. Moreover, four flavonoidal compounds were isolated to our knowledge for the first time from the peels and tested separately against different human cancer cell lines and the mode of action of the most potent compound has been determined. P. sativum ethyl acetate fraction possessed the highest scavenging activity (31.2%) as well as the most cytotoxic effect on breast carcinoma cell line. Apigenin proved to be the most potent tested compound on (MCF-7) and has no cytotoxic effect on normal human skin cell lines.