Anticancer and Cytotoxicity Activity of Native and Modified Black Rice Flour on Colon Cancer Cell Lines.

B Thanuja,Kibrom Meansbo Hadish,Kaliannan Durairaj,S Vijayanand,R Parimalavalli,Neveen Abdel-Raouf,Ibraheem Borie M Ibraheem,Essam Nageh Sholkamy,Reem Mohammed Alharbi

doi:10.1155/2022/8575026

Abstract

This study is intended to evaluate the cytotoxicity of native and dual-modified black rice flour against the colon cancer cell line (HCT116) and mouse embryo cell line (3T3-L1) by using the MTT assay. The modification techniques applied to prepare rice flour samples were enzymatic modification and heat moisture treatment. In this study, the IC50 of native black rice flour and modified black rice flour was 255.78 µg/mL and 340.85 µg/mL, respectively. The result confirms that the native black rice flour has significant cytotoxic and anticancer potential against human colon cancer cells. In addition, the IC50 of native black rice flour and modified black rice flour on the 3T3-L1 cell line was found to be 345.96 µg/mL and 1106.94 µg/mL, respectively. The results showed that the native black rice flour had weak cytotoxicity, and modified black rice flour was nontoxic in both the cell lines. The active component of phytochemicals present in black rice flour has a potential role in preventing colon cancer.

Highlights

IntroductionCancer is one of the health concerns
Worldwide, cancer is one of the health concerns
The precipitate was collected by centrifugation by washing twice with distilled water. en, it was oven-dried at 40°C for 25% of its moisture content. e samples were sealed in a container to store at room temperature for 24 hrs. en, for about one hour, the containers were placed in a hot air oven at 100°C. e flour was sieved to obtain a 100 mesh (150 μm) particle size and stored at 4°C. e modification methods applied were enzymatic modification and heat moisture treatment

Summary

Introduction

Cancer is one of the health concerns. One in eight deaths worldwide is due to cancer [1]. When we consider the risk factors, genetic defects account for only 5–10% of most cancers. More than 90% are due to modifiable risk factors such as environmental and lifestyle factors [2, 3]. E term “carcinogenesis” is a multistage process comprising phases such as initiation, promotion, and progression. E vital goal of chemoprevention is to block tumor progression [5]. Diet is recognized as a significant modulator in the development of colon cancer; high consumption of plant foods such as vegetables, fruits, and whole grains containing bioactive phytochemicals has been linked to a decreased risk of colon

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Conclusion