Abstract
Isaria cicadae, a medicinal food fungus, is a fruit from Paecilomyces cicadae. In this study, we purified ergosterol peroxide (EP) from the fermentation broth of P. cicadae and investigated its effects on renal cell carcinoma (RCC) cells, in vitro. EP was purified from P. cicadae fermentation broth. The human RCC cell line 786-0 was used to analyze the anticancer mechanism of EP and inhibit its effect on cancer cell proliferation, in vitro. EP with a validated structure showed a yield rate of 20.1 mg/L and a purity of 96%. EP significantly inhibited RCC cell growth and clone formation in vitro. In addition, EP suppressed the migration and invasion, triggered the apoptosis, and modulated the cell cycle of RCC cells, in a dose-dependent manner. It also downregulated β-catenin expression. EP could be routinely produced through P. cicadae. It fights RCC cells in vitro through multiple mechanisms, including suppressing cell growth, colonization, migration, and invasion, arresting the cell cycle, attenuating β-catenin pathways, and triggering apoptosis.
Highlights
Renal cell carcinoma (RCC) originates from the renal epithelium
Emerging evidence has pointed to the anti-tumor activity of Ergosterol peroxide (EP) in colorectal cancer, hepatocellular carcinoma, prostate cancer, myeloma, and leukemia [13,14,15,16], with known mechanisms like β-catenin signaling, apoptosis-promoting pathways, and anti-angiogenic pathways targeting JAK2/STAT3 signals, etc
Reports about EP obtained from P. cicadae fermentation, and its role in renal cancer tumors worldwide, are scant
Summary
Renal cell carcinoma (RCC) originates from the renal epithelium It accounts for more than 90% of cancers in the kidney and about 3% of all lethal, malignant, tumors [1]. Many effective anti-cancer formulas have been derived from these herbs, under the guidance of the traditional Chinese medicine (TCM) theory [5,6,7]. The anti-tumor effects of I. cicadae have been increasingly observed, such as the antiproliferative and pro-apoptosis properties in gynecological carcinoma cells [8]. Ergosterol peroxide (EP) is one of the major active ingredients in I. cicadae (molecular formula: C28H44O·C6H12O7). We aimed to purify EP from P. cicadae fermentation broth, explore its anti-RCC effects on a human RCC cell line, and elucidate the underlying mechanisms, at least partially in vitro. This study may provide evidence for EP application in renal cancer treatment
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
