Abstract

The Swedish Human Protein Atlas (HPA) program has been set up to allow for a systematic exploration of the human proteome using Antibody-Based Proteomics. This is accomplished by combining high-throughput generation of affinity-purified (mono-specific) antibodies with protein profiling in a multitude of tissues/celltypes assembled in tissue microarrays. A web-based annotation software has been developed to allow for a basic and rapid evaluation of immunoreactivity in tissues. Manual annotation of scanned images of tissue microarrays are performed on a virtual microscope over the internet by Indian pathologist annotators in Mumbai. Intensity, fraction of immunoreactive cells and subcellular localization is recorded for each given cell population. A text comment summarizing the characteristics for each antibody is added.

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