Abstract
Antibody against actin isolated from a human EBV-transformed lymphoblastoid B-cell line exerted an inhibitory effect on in vitro IgM secretion by a different lymphoblastoid B-cell line, LA350. This effect was dose dependent showing from 24-40% inhibition at a dilution of 1:100 and 68-80% inhibition at a dilution of 1:50. This effect was noted in the absence of changes in either total cell count or [3H]-thymidine incorporation and was reversed by co-incubation with purified rabbit thymus actin (100 micrograms/ml) but not bovine serum albumin at the same concentration. These data demonstrate regulation of immunoglobulin synthesis by antibodies against human lymphocyte derived actin in a lymphoblastoid B-cell line.
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