Year-end Sale % OFF 
Abstract
TSOL18 is a recombinant protein that has been shown in repeated experimental trials to be capable of protecting pigs against challenge infection with the cestode parasite Taenia solium. Antibodies raised by the vaccine are capable of killing the parasite in an in vitroculture and it is believed that antibody and complement-mediated killing of invading parasites is the major protective immune mechanism induced by vaccination with TSOL18. Investigations were undertaken to characterize whether the principal antibody specificities raised by TSOL18 in pigs were against linear or conformational determinants. TSOL18 was expressed in two truncated forms representing either the amino terminal portion or the carboxy terminal portion, with the two truncations overlapping in sequence by 25 amino acids. The original protein (designated TSOL18N−) and the two truncations (TSOL18N−-1 and TSOL18N−-2) were used in inhibition ELISA. TSOL18N− was shown to be capable of completely inhibiting the binding of pig anti-TSOL18N− antibodies to TSOL18N− in ELISA. However, neither TSOL18N−-1 nor TSOL18N−-2, either alone or when combined together, was capable of inhibiting any detectable amount of reactivity of pig anti-TSOL18N− antibodies with TSOL18N−. It is concluded that the dominant antibody specificities, and probably the host-protective specificities, of TSOL18 are conformational epitopes.
Highlights
Taenia solium is a zoonotic parasite prevalent in many developing countries of Asia, Africa and the Americas [1,2,3]
Transmission to pigs is through the ingestion of faeces from a human infected with the adult T. solium tapeworm, or items contaminated with such faeces
TSOL18N)-1-GST and TSOL18N)-2-GST were expressed in E. coli as soluble fusion proteins that were successfully purified on glutathione agarose and migrated according to their predicted sizes in SDS PAGE (Figure 1b)
Summary
Taenia solium is a zoonotic parasite prevalent in many developing countries of Asia, Africa and the Americas [1,2,3]. Transmission to pigs is through the ingestion of faeces from a human infected with the adult T. solium tapeworm, or items contaminated with such faeces. Control of the disease can be achieved by improvements in sanitary conditions concerning the disposal of human faeces and through prevention of pigs gaining access to human faeces. Efforts have been made to control this disease, but few countries have been able to eradicate or reduce the infection level using approaches such as anthelmintic treatment of humans or pigs, restriction of roaming pigs, health education or meat inspection. Failure to control T. solium cysticercosis using these approaches in the last decades has indicated that eradication of this zoonosis will be difficult to achieve [4]
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
