Abstract
The Alloantigenic Sites of α3α4α5(IV) Collagen: PATHOGENIC X-LINKED ALPORT ALLOANTIBODIES TARGET TWO ACCESSIBLE CONFORMATIONAL EPITOPES IN THE α5NC1 DOMAIN
Highlights
This allows the preparation of hy- From previous NMR studies it is known that A site metal brid alkaline phosphatases in which A and B sites of serves to coordinate the phosphate group in thenoncovalent each monomer contain two different species of metal Michaelis complex E .P, whereas any charge neutralization
We report here the properties of several mixed metal hybrids of alkaline the dominant influence in dephosphorylation of E-P phosphatase in which A and B sites contain different metal and may have a coordinated water molecule, which ions
As the phosphate is transferred to serine 102, its coordination site on A site metal ionwould become available to solvent
Summary
Is clear evidence for long range effects of subunit- Enzyme Preparation-Alkaline phosphatase was isolated from an subunit interactions, since metal ion and phosphate overproducing strain of E. coli,CW3747,by osmotic shock and binding at one active centeralters theenvironments of purified as previously described (4). Metal ion concentrations of reconstituted metalloenzyme were publication of this article were defrayed in part by the payment of. This article must be hereby marked "aduer- taining cadmium ions at A, B, and C sites; Z ~ I ( I I ) ~ AanPd Zn(II)JP, tisenent" in accordance with 18U.S.C. Section 1734 solelyto indicate alkaline phosphatase containing zinc ions at A sites alone or A and this fact. Mixed Metal Hybrids of Alkaline Phosphatase checked by flame atomic absorption using an IL157 spectrometer (Instrumentation Laboratories). T1 values are in the range 3-6 s, so flip angles of -30" were employed with repetition times of 1s
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