Abstract
Summary Cells of the cell line P3-J (Jijoye), derived from a patient with Burkitt's lymphoma, were exposed to coliphage T2. Protein isolated from spent culture medium suppressed plaque formation by T2, but not by coliphage T5. When the cultured cells had been exposed to T5, the protein fraction had a neutralizing activity to T5 but not to T2. This activity was reduced by treatment with dithiothreitol, mercaptoethanol, antihuman whole γ globulin serum, or anti-human IgM serum. Protein fractions from the spent media of P3-J cultures that had not been exposed to the test phage had no activity. The protein preparations from cultured human osteosarcoma or mesothelioma cells that had been similarly exposed to coliphages showed no such activity. These experiments strongly suggest production of a “specific” antibody, probably IgM, by P3-J cells stimulated by coliphages acting as antigens.
Published Version
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