Antibody Phage Display

Abstract

The fundamental success of many diagnostic platforms is subjected to the sensitivity and specificity of the biomolecules used for detection. One of the most common used biomolecules for diagnostics are antibodies. Antibodies are naturally occurring proteins found in circulation in the human body that protect the host from infections. However, recent advances in molecular biology have enabled the development of human monoclonal antibodies in vitro. Antibody phage display is a common approach used for the isolation and identification of human monoclonal antibodies that can be applied for diagnostic platforms. Antibody phage display utilizes bacteriophage particles to present the phenotype of the encoded antibody genotype on its surface. A selection process allows for enrichment of specific binders based on affinity selection. The development of recombinant human monoclonal antibodies in this manner also provides additional possibilities for engineering and modification for specific applications in diagnostics.