Antibody CD133 Biofunctionalization of Ammonium Acryloyldimethyltaurate and Vinylpyrrolidone Co-Polymer-Based Coating of the Vascular Implants.

Przemysław Sareło,Marlena Gąsior-Głogowska,Halina Podbielska,Marta Kopaczyńska,Magdalena Wawrzyńska,Wojciech Kałas,Maciej Duda,Edyta Wysokińska

doi:10.3390/ma13245634

Abstract

Current vascular stents, such as drug eluting stents (DES), have some serious drawbacks, like in stent restenosis and thrombosis. Therefore, other solutions are sought to overcome these post-implantations complications. These include the strategy of biofunctionalization of the stent surface with antibodies that facilitate adhesion of endothelial cells (ECs) or endothelial progenitor cells (EPCs). Rapid re-endothelialization of the surface minimizes the risk of possible complications. In this study, we proposed ammonium acryloyldimethyltaurate/vinylpyrrolidone co-polymer-based surface (AVC), which was mercaptosilanized in order to expose free thiol groups. The presence of free thiol groups allowed for the covalent attachment of CD133 antibodies by disulfide bridges formation between mercaptosilanized surface and cysteine of the protein molecule thiol groups. Various examinations were performed in order to validate the procedure, including attenuated total reflection–Fourier transform infrared spectroscopy (ATR-FTIR) and Fourier transform Raman spectroscopy (FT-Raman), atomic force microscopy (AFM) and scanning electron microscopy (SEM). By means of ATR-FTIR spectroscopy presence of the CD133 antibody within coating was confirmed. In vitro studies proved good biocompatibility for blood cells without induction of hemolytic response. Thus, proposed biofunctionalized CD133 antibody AVC surface has shown sufficient stability for adapting as cardiovascular implant coating and biocompatibility. According to conducted in vitro studies, the modified surface can be further tested for applications in various biological systems.

Highlights

Nowadays, numerous studies are carried out on coatings that are biofunctionalized with various biocompounds and biomolecules in order to obtain the coating specific properties
This process was monitored by means of FT-Raman spectroscopy
(3-mercaptopropyl)triethoxysilane reagent obtain steel discs surface by means of preparation ammonium acryloyldimethyltaurate/vinylpyrrolidone steel discs surface by means of preparation ammonium acryloyldimethyltaurate/vinylpyrrolidone exposed free thiolsthin groups the material surface—potentially formatted disulfide bridges were co-polymer-based film, on introduction of the

Summary

Introduction

Numerous studies are carried out on coatings that are biofunctionalized with various biocompounds and biomolecules in order to obtain the coating specific properties. The functionalized material surfaces with antibodies are of particular interest. Immobilized anti-epithelial cell adhesion antibodies have found application in circulating tumor cells capture to prevent metastases development [1] in endothelial progenitor cells capture, which differentiate into a mature endothelium, Materials 2020, 13, 5634; doi:10.3390/ma13245634 www.mdpi.com/journal/materials. Materials 2020, 13, 5634 increasing cardiovascular stents biocompatibility [2,3,4,5,6,7] in biosensor design and construction as well as diagnostic platforms with significantly improved detection efficiency [8,9]. The critically important factor to be considered still remains, like immobilization efficiency of a well-oriented antibody onto the surface to achieve high molecule loading with affinity retention [9,10]. The different surface antibody immobilization methods have been extensively studied. The physical adsorption [11,12], covalent bonding [11,12,13,14] or bio-affinity immobilization approach [15]

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Conclusion