Abstract
Antibody-based sensors permit the rapid and sensitive analysis of a range of pathogens and associated toxins. A critical assessment of the implementation of such formats is provided, with reference to their principles, problems and potential for ‘on-site’ analysis. Particular emphasis is placed on the detection of foodborne bacterial pathogens, such as Escherichia coli and Listeria monocytogenes, and additional examples relating to the monitoring of fungal pathogens, viruses, mycotoxins, marine toxins and parasites are also provided.
Highlights
Pathogenic bacterial, fungal and viral cells are ubiquitous in nature and pose a considerable risk to human and animal health, in addition to severely compromising the quality of agricultural produce (Table 1)
This review provides a comprehensive summary of the principles, problems and potential of using immunosensor-based analytical platforms for pathogen detection
These antigens are typically administered in the presence of a suitable adjuvant, and the immune response generated by the host after a series of immunisations can be determined by screening serial serum dilutions for recognition of the antigen in an enzyme-linked immunosorbent assay (ELISA)-based format
Summary
Pathogenic bacterial, fungal and viral cells are ubiquitous in nature and pose a considerable risk to human and animal health, in addition to severely compromising the quality of agricultural produce (Table 1). The monitoring of these microorganisms is of paramount importance for the prevention of nosocomial infections, the maintenance of general public health and for ensuring. The rapid detection and identification of a pathogen is essential, in particular where food samples with short shelf-lives are being analysed, or where the urgent administration of a suitable antimicrobial agent is required to treat a potential fatal infection. Developing suitable detection methods which permit accurate, rapid and sensitive analysis is essential for monitoring the distribution of pathogens and, most importantly, ensuring customer/patient safety. Human pathogen; causative agent of foodborne illness and producer of toxins, such as verocytoxin or ‘shiga-like’ toxin
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