Antibiotic Resistance in Vibrio spp. Causing Disease in Barramundi Fish (Lates Calcarifer) Eye

Abstract

The cultivation of barramundi fish (Lates calcarifer) is widely carried out because barramundi fish has a relatively high economic value. The disease in question is caused by Vibrio spp. bacteria, which are marine bacteria that often affect barramundi fish, and the treatment for the bacteria is antibiotics. Therefore, research was conducted to test several types of antibiotics and observe the bacterial resistance reaction to the administration of these antibiotics. The methods used in this study included media preparation, purification, and microscopic characterization, liquid media preparation, solid media preparation, and antibiotic sensitivity testing. Vibrio spp. are still sensitive to antibiotics at a rate of 61.27%, resistant at 18.14%, and intermediate at 20.59%. Bacterial isolate characteristics were divided into several types: Irregular, Punctiform, Round, and Round Umbonate. Irregular, Punctiform, Round, and Round Umbonate are still sensitive to antibiotics at rates ranging from 37.5% to 66.67%. Other results include intermediate and resistance with results ranging from 15.63% to 37.5% and 15.10% to 25.00%, respectively. The highest sensitivity results were found in antibiotics such as Gentamycin (98.94%), Co-Amoxiclave (86%), Tetracycline HCl (72.55%), Ciprofloxacin HCl (82.35%), Azithromycin (52.94%), and Doxycycline (54.90%). On the other hand, the highest intermediate and resistance results were found in chloramphenicol (52.94%) and Ampicillin (60.78%), respectively. Ampicillin is considered to be the antibiotic that the bacteria are resistant to because of its small inhibitory zone.