Susceptibility of Vibrio spp. from Viscera Organ and Flesh of Lates calcarifer Against Antibiotics

Abstract

Cultivation of Lates calcarifer is widely developed in Indonesia due to its high market demand and economic value. The biggest challenge in aquaculture is the diseases caused by pathogenic bacteria. The presence of pathogenic bacteria can disrupt the cultivation process and even lead to mass mortality in fish. Vibrio spp. is one of the pathogenic bacteria found in the cultivation of white snapper. The growth and development of Vibrio spp. bacteria can be controlled by the use of antibiotics. However, excessive and improper use of antibiotics can trigger the development of antibiotic resistance. Therefore, resistance testing is necessary to determine the level of resistance of Vibrio spp. bacteria from the viscera organ and flesh of L. calcarifer to 8 types of antibiotics, namely Gentamicin, Co-Amoxiclav, Tetracycline HCl, Ciprofloxacin HCl, Ampicillin, Chloramphenicol, Azithromycin, and Doxycycline. Resistance testing of Vibrio spp. bacteria from the viscera organ and flesh of L. calcalifer to these 8 types of antibiotics is carried out using the disc diffusion method. Vibrio spp. bacteria from the viscera organ of fish have resistance to Ampicillin and Co-Amoxiclav with a resistance percentage of 35.7%, while Vibrio spp. bacteria from the flesh of L. calcarifer have resistance to Ampicillin and Azithromycin with a resistance percentage of 57.1%. Vibrio spp. bacteria from the viscera organ and flesh are sensitive to Gentamicin with a percentage of 100%. Vibrio spp. bacteria from the viscera organ and flesh of L. calcarifer have different resistance and sensitivity to various types of antibiotics. Overall, the Vibrio spp. obtained in this study are still sensitive to antibiotics.