Antibiotic resistance genes removals in stormwater bioretention cells with three kinds of environmental conditions

Abstract

Recently, increasing attention has been paid to antibiotic resistance genes (ARGs) in stormwater runoff. However, there is still no available literature about ARGs removals through stormwater bioretention cells. Batch experiments were conducted to investigate target ARGs (blaTEM, tetR and aphA) removals under three environmental conditions, including substrate (weight ratios of sand to soil), hydraulic loading rate (HLR) and submerged area depth. The target ARGs removals were the largest (more than 5 log in the bottom outlets) in bioretention cells with 8:2 ratio of sand to soil, HLR 0.044 cm3/cm2/min and 150 mm of submerged area depth. The proportion for both iARGs and eARGs had little effect on target ARGs removals (expect extracellular blaTEM), although distributions of target ARGs were different in substrate layers. Adsorption behavior tests indicated that both kinetics and isotherms of target ARGs adsorption by biofilms were more suitable to explain their best removals for bioretention cells with 8:2 ratio of sand to soil than that by substrate. At phylum and genus levels, there were respectively 6 dominant microflora related significantly to target ARGs levels, and their relationships changed obviously under different environmental conditions, suggesting that regulating the dominant microflora (like Verrucomicrobia and Actinobacteria) could be feasible to change ARGs removals.