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Abstract
Previous studies in our laboratory have questioned the efficacy of antibiotic impregnated bone cement to prevent staphylococcal colonization of orthopaedic implants following surgery. Teicoplanin a glycopeptide antibiotic with excellent anti-staphylococcal activity was added to polymethylmethacrylate in concentrations ranging from 2-5 wt%. Five staphylococcal strains recovered from orthopaedic implant infections were chosen for study: 2-Staphylococcus aureus and 3-Staphylococcus eoidermidis. Two of the staphylococcal isolates were identified as producing an exopolysaccharide slime following alcian blue staining with lysine. The in vitro release of antibiotic from the resin surface was determined by biological assay at 0, 12, 24, 48, 96 and 10 days post-study. Mean teicoplanin concentrations ranged from 200 to 430 ug/ml at 0 hour and 22 to 50 ug/ml at 10 days post-study. Teicoplanin impregnated and drug free resin controls were exposed to standardized inoculum (5.0 log10 colony forming units/ml) of the staphylococcal clinical isolates and incubated at 35°C in physiologic buffered saline with 0.25% dextrose.
Published Version
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Schedule a callMore From: Proceedings, annual meeting, Electron Microscopy Society of America
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