Antibiogram Pattern of Common Plant Leaves against E. coli, Staphylococcus aureus and Whole Milk Culture

Abstract

The in vitro efficacy of ethanolic and aqueous extract of selected plants namely Neem (Azadirachta indica), Guava (Psidium guajava), Lemongrass (Cymbopogon citratus), Mango (Mangifera indica), Tulsi (Ocimum sanctum) and Seasum (Dalbergia sissoo) leaves was studied against E. coli, Staphylococcus aureus and whole milk culture. The antibiogram revealed that S. aureus isolates were 100% susceptible to ethanolic extracts of only Neem and Guava in addition to standard drug Streptopenicillin followed by Lemongrass that showed 60 % sensitivity. The percent sensitivity of ethanolic extract of these plants against isolates of E. coli was 60, 40, 40, 0, 0 and 0, respectively. In whole milk culture the sensitivity percent was 100% for Neem, Guava, Lemon grass in comparison to 40% for Mango leaves and 0 % each for Tulsi and Seasum leaves. The aqueous extract of Neem, Lemongrass and Mango leaves did not show any activity against the isolates of S. aureus (0% sensitivity). All the leaf extracts were inactive against E. coli (0% sensitivity). The extract of Neem exhibited sensitivity against 40% isolates of whole milk culture.