Antibiogram characterization and putative virulence genes in Aeromonas species isolated from pig fecal samples.

Abstract

Aeromonas species are broadly distributed in nature and agricultural environments and have been isolated from feces, bedding, and drinking water of healthy pigs. We assessed the incidence, virulence properties, and antimicrobial resistance profile of Aeromonas spp., isolated from pig feces. Antibiogram was done using the disc diffusion methods, and polymerase chain reaction was used for the detection of putative virulence genes. Identification of isolates revealed three phenotypic species with percentage distribution as follows: Aeromonas hydrophila 23 (45.1%), Aeromonas caviae 16 (31.4%), and Aeromonas sobria 12 (23.5%). All Aeromonas isolates in the study were absolutely susceptible to cefotaxime and resistant to penicillin. A. cavaie and A. sobria demonstrated absolute susceptibility against ciprofloxacin and streptomycin. Aeromonas species showed varied susceptibility to cephalothin as follows: A. hydrophila 78.3%, A. cavaie 93.7%, and A. sobria 91.7%. The percentage distribution of virulence genes among Aeromonas isolates were as follows: Aerolysin (aer) 74.5%, flagellin gene (fla) 68.6%, cytotoxin (hly A) 43.1%, lipase (lip) 39.2%, enterotoxic activities (ast) 31.3%, and cytotonic gene (alt) 13.7%. Reports from this study shows that Aeromonas species isolated from pig fecal samples are multi-drug resistant and possess virulence potential which may result to possible risk of human or animal infection and likely contamination of food and water from this sources.